Eppendorf - 5402 Manual Updated

The Eppendorf 5402 is a versatile pipette designed for a wide range of laboratory applications. Some of its key features include:

Typically holds 18 tubes (1.5 mL or 2.0 mL) using the standard fixed-angle rotor.

For safe operation, always load the rotor symmetrically. Opposing tubes must be the same type and filled with an equal mass .

Press a final time to reach the Temperature display. Set the required temperature for your protocol. Initiating and Terminating the Cycle

Adjust the speed knob or arrow buttons until the desired RPM or g-force is shown on the digital display. eppendorf 5402 manual updated

Always ensure the rotor is properly tightened onto the drive shaft.

0 to 99 minutes or a "momentary" button for short pulses. Essential Operating Instructions 1. Pre-Cooling (Fast Cool)

Opposing tubes must contain equal volumes of liquid. If running an odd number of samples, create a balance tube filled with water to match the weight of the sample tube exactly.

If the unit was previously run at low temperatures, wipe away any pooled water to prevent icing around the cooling coils. The Eppendorf 5402 is a versatile pipette designed

| | Original Instruction | Updated Instruction (Rev. 5+) | |-----------|--------------------------|------------------------------------| | Rotor inspection | Visual check monthly | Visual + dye penetrant test every 6 months | | Lid lock test | Not mentioned | Perform microswitch continuity test before each use | | Temperature calibration | User not advised | Use external Pt100 sensor quarterly; offset adjustable via rear panel | | Emergency lid release | Mechanical pull cord | Mechanical pull cord + magnetic override code (in addendum) |

Visit the official (eppendorf.com).

Eppendorf has updated general centrifuge care protocols that apply to legacy models like the 5402 to ensure operational safety: Centrifuge 5420 - Eppendorf

– Some early manual versions had vague schematics for the refrigerant type (R134a vs. older CFC-based coolants). Updated addendums clarify that the 5402 uses R134a, and they provide corrected voltage tolerances (±10% of 230V for EU models; ±10% of 120V for NA models). Opposing tubes must be the same type and

. Because it is a legacy model, updated "deep reports" or manuals often focus on maintenance, troubleshooting common error codes, and finding compatible replacement parts. Core Specifications & Features

: Ensure the rotor is properly seated on the drive shaft and the knurled nut is tightened securely. Lid Mechanism

Many university environmental health and safety (EHS) departments host public PDF copies of legacy equipment manuals.

The is a legacy refrigerated benchtop microcentrifuge known for its reliability in molecular biology applications. While newer models like the Centrifuge 5420 have succeeded it, maintaining the 5402 requires adhering to original technical specifications and updated maintenance standards. Core Technical Specifications Maximum Speed: 14,000 RPM (approx. 16,000 x g). Temperature Range: -4∘Cnegative 4 raised to the composed with power C 40∘C40 raised to the composed with power C , providing stable cooling for sensitive samples.

This usually indicates a fault with the temperature sensor or a refrigeration fluid leak. Turn off the machine, unplug it from the wall, and contact a certified technician. 6. Finding and Downloading the Updated Manual